clontech dropout plates Search Results


synthetic dropout  (TaKaRa)
95
TaKaRa synthetic dropout
Synthetic Dropout, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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synthetic dropout selection agar plates  (TaKaRa)
94
TaKaRa synthetic dropout selection agar plates
Synthetic Dropout Selection Agar Plates, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sc minimal dropout  (TaKaRa)
86
TaKaRa sc minimal dropout
Sc Minimal Dropout, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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synthetic dropout sd agar plates  (TaKaRa)
94
TaKaRa synthetic dropout sd agar plates
Synthetic Dropout Sd Agar Plates, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aureobasidin a  (TaKaRa)
96
TaKaRa aureobasidin a
(A) Structure of PRH1 promoter and the fragments used in the CHIP-qPCR assay. AuxREs are indicated by red squares, and black lines show the promoter regions containing the AuxREs used in this assay. NC: negative control. AuxREs: auxin response elements. (B) ARF7 transactivates the PRH1 promoter in A . thaliana leaf protoplasts. The left hand panel is a schematic of the effector ( 35S :: ARF7 ) and reporter ( PRH1pro : LUC ) constructs. The empty vector pBI221 was used as a negative control; the right hand panel shows the ratio of ARF7 drived LUC and the empty vector (negative control) to 35S promoter drived REN respectively. LUC: firefly luciferase activity, REN: renilla luciferase activity. Values shown as means±SE, three biological replicates in the experiment. *: means differ significantly ( P <0.05) from the negative control. (C) ARF7 is associated with the PRH1 promoter according to a CHIP-qPCR assay. Chromatin isolated from a plant harboring 35S :: MYC-ARF7 and a WT mock control was immunoprecipitated with anti-MYC antibody following the amplification of regions P1, P2 and P3. The coding region segment NC was used as the negative control. The ChIP signal represents the ratio of bound promoter fragments (P1-P3) after immunoprecipitation to total input without immunoprecipitation. Values shown as means±SE, three biological replicates in the experiment. **, ***: means differ significantly ( P <0.01, P< 0.001) from the WT control. (D) Physical interaction of ARF7 with the PRH1 promoter according to a Y1H assay. The plasmid pGADT7-ARF7 was introduced into Y1H Gold cells harboring the reporter gene PRH1pro : AbAr and the cells were grown on SD/-Ura-Leu medium in the presence of 30 or 50 ng/mL <t>aureobasidin</t> <t>A</t> (AbA). The empty vector pGADT7 was used as a negative control.
Aureobasidin A, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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clontech dropout plates  (TaKaRa)
93
TaKaRa clontech dropout plates
(A) Structure of PRH1 promoter and the fragments used in the CHIP-qPCR assay. AuxREs are indicated by red squares, and black lines show the promoter regions containing the AuxREs used in this assay. NC: negative control. AuxREs: auxin response elements. (B) ARF7 transactivates the PRH1 promoter in A . thaliana leaf protoplasts. The left hand panel is a schematic of the effector ( 35S :: ARF7 ) and reporter ( PRH1pro : LUC ) constructs. The empty vector pBI221 was used as a negative control; the right hand panel shows the ratio of ARF7 drived LUC and the empty vector (negative control) to 35S promoter drived REN respectively. LUC: firefly luciferase activity, REN: renilla luciferase activity. Values shown as means±SE, three biological replicates in the experiment. *: means differ significantly ( P <0.05) from the negative control. (C) ARF7 is associated with the PRH1 promoter according to a CHIP-qPCR assay. Chromatin isolated from a plant harboring 35S :: MYC-ARF7 and a WT mock control was immunoprecipitated with anti-MYC antibody following the amplification of regions P1, P2 and P3. The coding region segment NC was used as the negative control. The ChIP signal represents the ratio of bound promoter fragments (P1-P3) after immunoprecipitation to total input without immunoprecipitation. Values shown as means±SE, three biological replicates in the experiment. **, ***: means differ significantly ( P <0.01, P< 0.001) from the WT control. (D) Physical interaction of ARF7 with the PRH1 promoter according to a Y1H assay. The plasmid pGADT7-ARF7 was introduced into Y1H Gold cells harboring the reporter gene PRH1pro : AbAr and the cells were grown on SD/-Ura-Leu medium in the presence of 30 or 50 ng/mL <t>aureobasidin</t> <t>A</t> (AbA). The empty vector pGADT7 was used as a negative control.
Clontech Dropout Plates, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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synthetic dropout sd plates  (TaKaRa)
86
TaKaRa synthetic dropout sd plates
(A) Structure of PRH1 promoter and the fragments used in the CHIP-qPCR assay. AuxREs are indicated by red squares, and black lines show the promoter regions containing the AuxREs used in this assay. NC: negative control. AuxREs: auxin response elements. (B) ARF7 transactivates the PRH1 promoter in A . thaliana leaf protoplasts. The left hand panel is a schematic of the effector ( 35S :: ARF7 ) and reporter ( PRH1pro : LUC ) constructs. The empty vector pBI221 was used as a negative control; the right hand panel shows the ratio of ARF7 drived LUC and the empty vector (negative control) to 35S promoter drived REN respectively. LUC: firefly luciferase activity, REN: renilla luciferase activity. Values shown as means±SE, three biological replicates in the experiment. *: means differ significantly ( P <0.05) from the negative control. (C) ARF7 is associated with the PRH1 promoter according to a CHIP-qPCR assay. Chromatin isolated from a plant harboring 35S :: MYC-ARF7 and a WT mock control was immunoprecipitated with anti-MYC antibody following the amplification of regions P1, P2 and P3. The coding region segment NC was used as the negative control. The ChIP signal represents the ratio of bound promoter fragments (P1-P3) after immunoprecipitation to total input without immunoprecipitation. Values shown as means±SE, three biological replicates in the experiment. **, ***: means differ significantly ( P <0.01, P< 0.001) from the WT control. (D) Physical interaction of ARF7 with the PRH1 promoter according to a Y1H assay. The plasmid pGADT7-ARF7 was introduced into Y1H Gold cells harboring the reporter gene PRH1pro : AbAr and the cells were grown on SD/-Ura-Leu medium in the presence of 30 or 50 ng/mL <t>aureobasidin</t> <t>A</t> (AbA). The empty vector pGADT7 was used as a negative control.
Synthetic Dropout Sd Plates, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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synthetic dropout selection agar plates (sd/-leu/-trp/-ade/-his/5-bromo-4-chloro-3-indolyl- -d-galactopyranoside  (Becton Dickinson)
90
Becton Dickinson synthetic dropout selection agar plates (sd/-leu/-trp/-ade/-his/5-bromo-4-chloro-3-indolyl- -d-galactopyranoside
(A) Structure of PRH1 promoter and the fragments used in the CHIP-qPCR assay. AuxREs are indicated by red squares, and black lines show the promoter regions containing the AuxREs used in this assay. NC: negative control. AuxREs: auxin response elements. (B) ARF7 transactivates the PRH1 promoter in A . thaliana leaf protoplasts. The left hand panel is a schematic of the effector ( 35S :: ARF7 ) and reporter ( PRH1pro : LUC ) constructs. The empty vector pBI221 was used as a negative control; the right hand panel shows the ratio of ARF7 drived LUC and the empty vector (negative control) to 35S promoter drived REN respectively. LUC: firefly luciferase activity, REN: renilla luciferase activity. Values shown as means±SE, three biological replicates in the experiment. *: means differ significantly ( P <0.05) from the negative control. (C) ARF7 is associated with the PRH1 promoter according to a CHIP-qPCR assay. Chromatin isolated from a plant harboring 35S :: MYC-ARF7 and a WT mock control was immunoprecipitated with anti-MYC antibody following the amplification of regions P1, P2 and P3. The coding region segment NC was used as the negative control. The ChIP signal represents the ratio of bound promoter fragments (P1-P3) after immunoprecipitation to total input without immunoprecipitation. Values shown as means±SE, three biological replicates in the experiment. **, ***: means differ significantly ( P <0.01, P< 0.001) from the WT control. (D) Physical interaction of ARF7 with the PRH1 promoter according to a Y1H assay. The plasmid pGADT7-ARF7 was introduced into Y1H Gold cells harboring the reporter gene PRH1pro : AbAr and the cells were grown on SD/-Ura-Leu medium in the presence of 30 or 50 ng/mL <t>aureobasidin</t> <t>A</t> (AbA). The empty vector pGADT7 was used as a negative control.
Synthetic Dropout Selection Agar Plates (Sd/ Leu/ Trp/ Ade/ His/5 Bromo 4 Chloro 3 Indolyl D Galactopyranoside, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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synthetic dropout nutrient medium  (TaKaRa)
86
TaKaRa synthetic dropout nutrient medium
(A) Structure of PRH1 promoter and the fragments used in the CHIP-qPCR assay. AuxREs are indicated by red squares, and black lines show the promoter regions containing the AuxREs used in this assay. NC: negative control. AuxREs: auxin response elements. (B) ARF7 transactivates the PRH1 promoter in A . thaliana leaf protoplasts. The left hand panel is a schematic of the effector ( 35S :: ARF7 ) and reporter ( PRH1pro : LUC ) constructs. The empty vector pBI221 was used as a negative control; the right hand panel shows the ratio of ARF7 drived LUC and the empty vector (negative control) to 35S promoter drived REN respectively. LUC: firefly luciferase activity, REN: renilla luciferase activity. Values shown as means±SE, three biological replicates in the experiment. *: means differ significantly ( P <0.05) from the negative control. (C) ARF7 is associated with the PRH1 promoter according to a CHIP-qPCR assay. Chromatin isolated from a plant harboring 35S :: MYC-ARF7 and a WT mock control was immunoprecipitated with anti-MYC antibody following the amplification of regions P1, P2 and P3. The coding region segment NC was used as the negative control. The ChIP signal represents the ratio of bound promoter fragments (P1-P3) after immunoprecipitation to total input without immunoprecipitation. Values shown as means±SE, three biological replicates in the experiment. **, ***: means differ significantly ( P <0.01, P< 0.001) from the WT control. (D) Physical interaction of ARF7 with the PRH1 promoter according to a Y1H assay. The plasmid pGADT7-ARF7 was introduced into Y1H Gold cells harboring the reporter gene PRH1pro : AbAr and the cells were grown on SD/-Ura-Leu medium in the presence of 30 or 50 ng/mL <t>aureobasidin</t> <t>A</t> (AbA). The empty vector pGADT7 was used as a negative control.
Synthetic Dropout Nutrient Medium, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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◦c on sc ura plate  (TaKaRa)
95
TaKaRa ◦c on sc ura plate
(A) Structure of PRH1 promoter and the fragments used in the CHIP-qPCR assay. AuxREs are indicated by red squares, and black lines show the promoter regions containing the AuxREs used in this assay. NC: negative control. AuxREs: auxin response elements. (B) ARF7 transactivates the PRH1 promoter in A . thaliana leaf protoplasts. The left hand panel is a schematic of the effector ( 35S :: ARF7 ) and reporter ( PRH1pro : LUC ) constructs. The empty vector pBI221 was used as a negative control; the right hand panel shows the ratio of ARF7 drived LUC and the empty vector (negative control) to 35S promoter drived REN respectively. LUC: firefly luciferase activity, REN: renilla luciferase activity. Values shown as means±SE, three biological replicates in the experiment. *: means differ significantly ( P <0.05) from the negative control. (C) ARF7 is associated with the PRH1 promoter according to a CHIP-qPCR assay. Chromatin isolated from a plant harboring 35S :: MYC-ARF7 and a WT mock control was immunoprecipitated with anti-MYC antibody following the amplification of regions P1, P2 and P3. The coding region segment NC was used as the negative control. The ChIP signal represents the ratio of bound promoter fragments (P1-P3) after immunoprecipitation to total input without immunoprecipitation. Values shown as means±SE, three biological replicates in the experiment. **, ***: means differ significantly ( P <0.01, P< 0.001) from the WT control. (D) Physical interaction of ARF7 with the PRH1 promoter according to a Y1H assay. The plasmid pGADT7-ARF7 was introduced into Y1H Gold cells harboring the reporter gene PRH1pro : AbAr and the cells were grown on SD/-Ura-Leu medium in the presence of 30 or 50 ng/mL <t>aureobasidin</t> <t>A</t> (AbA). The empty vector pGADT7 was used as a negative control.
◦C On Sc Ura Plate, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Structure of PRH1 promoter and the fragments used in the CHIP-qPCR assay. AuxREs are indicated by red squares, and black lines show the promoter regions containing the AuxREs used in this assay. NC: negative control. AuxREs: auxin response elements. (B) ARF7 transactivates the PRH1 promoter in A . thaliana leaf protoplasts. The left hand panel is a schematic of the effector ( 35S :: ARF7 ) and reporter ( PRH1pro : LUC ) constructs. The empty vector pBI221 was used as a negative control; the right hand panel shows the ratio of ARF7 drived LUC and the empty vector (negative control) to 35S promoter drived REN respectively. LUC: firefly luciferase activity, REN: renilla luciferase activity. Values shown as means±SE, three biological replicates in the experiment. *: means differ significantly ( P <0.05) from the negative control. (C) ARF7 is associated with the PRH1 promoter according to a CHIP-qPCR assay. Chromatin isolated from a plant harboring 35S :: MYC-ARF7 and a WT mock control was immunoprecipitated with anti-MYC antibody following the amplification of regions P1, P2 and P3. The coding region segment NC was used as the negative control. The ChIP signal represents the ratio of bound promoter fragments (P1-P3) after immunoprecipitation to total input without immunoprecipitation. Values shown as means±SE, three biological replicates in the experiment. **, ***: means differ significantly ( P <0.01, P< 0.001) from the WT control. (D) Physical interaction of ARF7 with the PRH1 promoter according to a Y1H assay. The plasmid pGADT7-ARF7 was introduced into Y1H Gold cells harboring the reporter gene PRH1pro : AbAr and the cells were grown on SD/-Ura-Leu medium in the presence of 30 or 50 ng/mL aureobasidin A (AbA). The empty vector pGADT7 was used as a negative control.

Journal: PLoS Genetics

Article Title: PRH1 mediates ARF7-LBD dependent auxin signaling to regulate lateral root development in Arabidopsis thaliana

doi: 10.1371/journal.pgen.1008044

Figure Lengend Snippet: (A) Structure of PRH1 promoter and the fragments used in the CHIP-qPCR assay. AuxREs are indicated by red squares, and black lines show the promoter regions containing the AuxREs used in this assay. NC: negative control. AuxREs: auxin response elements. (B) ARF7 transactivates the PRH1 promoter in A . thaliana leaf protoplasts. The left hand panel is a schematic of the effector ( 35S :: ARF7 ) and reporter ( PRH1pro : LUC ) constructs. The empty vector pBI221 was used as a negative control; the right hand panel shows the ratio of ARF7 drived LUC and the empty vector (negative control) to 35S promoter drived REN respectively. LUC: firefly luciferase activity, REN: renilla luciferase activity. Values shown as means±SE, three biological replicates in the experiment. *: means differ significantly ( P <0.05) from the negative control. (C) ARF7 is associated with the PRH1 promoter according to a CHIP-qPCR assay. Chromatin isolated from a plant harboring 35S :: MYC-ARF7 and a WT mock control was immunoprecipitated with anti-MYC antibody following the amplification of regions P1, P2 and P3. The coding region segment NC was used as the negative control. The ChIP signal represents the ratio of bound promoter fragments (P1-P3) after immunoprecipitation to total input without immunoprecipitation. Values shown as means±SE, three biological replicates in the experiment. **, ***: means differ significantly ( P <0.01, P< 0.001) from the WT control. (D) Physical interaction of ARF7 with the PRH1 promoter according to a Y1H assay. The plasmid pGADT7-ARF7 was introduced into Y1H Gold cells harboring the reporter gene PRH1pro : AbAr and the cells were grown on SD/-Ura-Leu medium in the presence of 30 or 50 ng/mL aureobasidin A (AbA). The empty vector pGADT7 was used as a negative control.

Article Snippet: Transformants were grown on SD/-Leu-Ura dropout plates containing different concentrations of aureobasidin A (CAT#630499, Clontech, USA).

Techniques: Negative Control, Construct, Plasmid Preparation, Luciferase, Activity Assay, Isolation, Immunoprecipitation, Amplification, Y1H Assay